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Mice were anesthetized by inhaling a mixture of 1 L/min oxygen and 1%1.5% isoflurane via a mask, while maintaining normal respiration

BiSpekDAb engineered by fusing anti-IL-23 domain antibody (VHH) and anti-TNF- domain antibody (VHH) to the 3rd domain of human serum albumin (3dHSA), using flexible linkers (GGGGS)

Future studies should focus on more molecular studies as well as RCTs with heterogeneous study designs and large samples in order to elucidate the mechanisms of the KD in tumor therapy and to evaluate the application of the KD in the clinical setting